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NR-12211   pMo90, Suicide Plasmid for Allelic Exchange in Burkholderia spp.
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: pMo90, Suicide Plasmid for Allelic Exchange in Burkholderia spp.
Organism: Burkholderia spp.
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: A Vazquez-Torres
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-12211 is a mobilizable suicide plasmid, pMo90, for allelic exchange in Burkholderia species (GenBank: FJ267588). Plasmid pMo90 was deposited cloned into host Escherichia coli (E. coli) TOP10 cells with no insert. After transformation into a commercially available chemically competent strain of E. coli, pMo90 was extracted using a QIAGEN® Plasmid Mega Kit.

Note: Sequencing results revealed that all significant elements of the vector described in the literature are present in NR-12211. The resulting size of the plasmid is 4.394 kilobases. The plasmid map and the complete plasmid sequence are provided on the Certificate of Analysis for NR-12211.

Each vial of NR-12211 contains approximately 1 µg of plasmid DNA in TE buffer.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: pMo90, Suicide Plasmid for Allelic Exchange in Burkholderia spp., NR-12211."
Additional Information: Life Technologies Limited Use Label License
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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