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NR-12214   pMo168, Replicative Plasmid for Allelic Exchange in Burkholderia spp.
(Plasmid/Vectors)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: pMo168, Replicative Plasmid for Allelic Exchange in Burkholderia spp.
Organism: Burkholderia spp.
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: A Vazquez-Torres
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-12214 is a mobilizable replicative plasmid, pMo168, for allelic exchange in Burkholderia species (GenBank: EU862244). Plasmid pMo168 was deposited cloned into host Escherichia coli (E. coli) JM109 cells with no insert. After transformation into a commercially available chemically competent strain of E. coli, pMo168 was extracted using a QIAGEN® Plasmid Mega Kit.

Note: Sequencing results revealed that all significant elements of the vector described in the literature are present in NR-12214. The resulting size of the plasmid is 7.111 kilobases. The plasmid map and the complete plasmid sequence are provided on the Certificate of Analysis for NR-12214.

Each vial of NR-12214 contains approximately 1 µg of plasmid DNA in TE buffer.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: pMo168, Replicative Plasmid for Allelic Exchange in Burkholderia spp., NR-12214."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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