Contact Us   Having technical issues or questions regarding this product?
Please Contact Us.
BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-13280   Plasmid pMRLB.7 Containing Gene Rv3875 (Protein Esat6) from Mycobacterium tuberculosis
(Plasmid/Vectors)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Plasmid pMRLB.7 Containing Gene Rv3875 (Protein Esat6) from Mycobacterium tuberculosis
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: -20°C or colder
Contributor: NIH - TB Vaccine Testing and Research Materials Contract
Comments:
NR-13280 is no longer available. Plasmid pMRLB.7 Containing Gene Rv3875 (Protein Esat6) from Mycobacterium tuberculosis, is now available as NR-36431. NR-36431 contains additional coding sequences that result in a more stable protein.

NR-13280 is a recombinant expression vector containing Mycobacterium tuberculosis gene Rv3875, which encodes the early secretory antigenic target Esat6, also known as esxA. Gene Rv3875 was amplified by PCR and cloned into pET23b for expression in Escherichia coli. The gene was cloned without a signal sequence. The expressed protein is histidine-tagged and has an observed molecular weight of 10 kDa. The expected purified protein yield from a one liter culture is approximately 5 mg.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pMRLB.7 Containing Gene Rv3875 (Protein Esat6) from Mycobacterium tuberculosis, NR-13280."
Publications Citing
this Reagent:
Eitson, J. L., et al. "Mycobacterial Shuttle Vectors Designed for High-Level Protein Expression in Infected Macrophages." Appl. Environ. Microbiol. 78 (2012): 6829-6837. PubMed: 22820329.
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-36431
Plasmid pMRLB.7 Containing Gene Rv3875 (Protein Esat6) from Mycobacterium tuberculosis
Return to Top


Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.


Back to My Search