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NR-13288   Plasmid pMRLB.24 Containing Gene Rv0577 from Mycobacterium tuberculosis
(Plasmid/Vectors)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Plasmid pMRLB.24 Containing Gene Rv0577 from Mycobacterium tuberculosis
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit for this item is 2 per year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-13288 is a recombinant expression vector containing Mycobacterium tuberculosis gene Rv0577, which encodes a conserved hypothetical protein, TB27.3. Gene Rv0577 was amplified by PCR and cloned into pET23b for expression in Escherichia coli. The gene was cloned with a signal sequence. The expressed protein is histidine-tagged and has an observed molecular weight of 27 kDa.

Note: Plasmid pMRLB.24 contains the gene required for ampicillin (Ap) resistance. The recommended concentration of Ap in culture is 130 µg/mL.

Each vial contains 1 µg of plasmid DNA in 10 mM Tris-HCl, pH 7.5.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pMRLB.24 Containing Gene Rv0577 from Mycobacterium tuberculosis, NR-13288."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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