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NR-13307   Plasmid pMRLB.57 Containing Gene Rv0379 (Protein SecE2) from Mycobacterium tuberculosis for Expression in Mycobacterium smegmatis
(Plasmid/Vectors)
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Description: Plasmid pMRLB.57 Containing Gene Rv0379 (Protein SecE2) from Mycobacterium tuberculosis for Expression in Mycobacterium smegmatis
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit for this item is 2 per year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-13307 is a recombinant expression vector containing Mycobacterium tuberculosis gene Rv0379, which encodes a putative transport protein, SecE2. Gene Rv0379 was amplified by PCR and cloned into pVV16 for expression in Mycobacterium smegmatis. The gene was cloned with a signal sequence. The expressed protein is histidine-tagged and has an observed molecular weight of 8 kDa.

Note: Plasmid pMRLB.57 contains the gene required for kanamycin resistance. The recommended concentration of kanamycin in culture is 50 µg/mL.

Each vial contains approximately 1 µg of plasmid DNA in 10 mM Tris-HCl, pH 8.5. Lot 07.EC.05.23 contains approximately 1 µg of plasmid DNA in 10 mM Tris-HCl, pH 7.5.
Citations: Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infection Research Resources Repository, NIAID, NIH: Plasmid pMRLB.57 Containing Gene Rv0379 (Protein SecE2) from Mycobacterium tuberculosis for Expression in Mycobacterium smegmatis, NR-13307."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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