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NR-13403   Shuttle Vector pMCSU1 for Gene Expression in Mycobacterium tuberculosis and Escherichia coli
(Plasmid/Vectors)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Shuttle Vector pMCSU1 for Gene Expression in Mycobacterium tuberculosis and Escherichia coli
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit for this item is 2 per year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-13403 is a shuttle vector that can be used for gene expression in either Escherichia coli or Mycobacterium tuberculosis. The pMCSU1 vector contains origins of replication for both organisms, Escherichia coli bacteriophage λ attR sites, a Mycobacterium BCG hsp60 promoter region, as well as the genes that confer resistance to kanamycin (Km), hygromycin B (Hyg) and chloramphenicol (Cm). The recommended antibiotic concentrations in culture are 50 µg/mL Km or 200 µg/mL Hyg.

Each vial contains approximately 1 µg of plasmid DNA in 10 mM Tris-HCl, pH ~ 8.0.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Shuttle Vector pMCSU1 for Gene Expression in Mycobacterium tuberculosis and Escherichia coli, NR-13403."
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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