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NR-28918   Plasmid Containing 18S Ribosomal RNA Gene Fragment from Babesia bigemina
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Description: Plasmid Containing 18S Ribosomal RNA Gene Fragment from Babesia bigemina
Organism: Babesia bigemina
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: FD Guerrero
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

A 582 base pair fragment of the 18S ribosomal RNA gene from Babesia bigemina (B. bigemina) was amplified by PCR and cloned into vector pPCR-Script Amp SK(+) (Agilent Technologies). The resulting plasmid, NR-28918, may be used in PCR assays for the detection of B. bigemina in cattle ticks or cattle blood. The plasmid was produced in One Shot® TOP10 chemically competent Escherichia coli (Invitrogen™) and extracted using a QIAGEN® EndoFree® Plasmid Maxi Kit.

Note: NR-28918 contains the gene required for carbenicillin (Ca) resistance. The recommended concentration of Ca in culture is 100 µg/mL.

Each vial contains 0.7 µg to 1.5 µg of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 8.0).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid Containing 18S Ribosomal RNA Gene Fragment from Babesia bigemina, NR-28918."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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