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NR-3025   Plasmid pETMPOX/B5RoΔTM Containing the B6R Gene from Monkeypox Virus, Zaire 79
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Description: Plasmid pETMPOX/B5RoΔTM Containing the B6R Gene from Monkeypox Virus, Zaire 79
Organism: Monkeypox virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: G Franchini
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The monkeypox B6R gene (GenBank AY160189) was amplified from plasmid pMPOX/B5Ro by PCR and subcloned into the prokaryotic expression vector pET26b (Novagen®), which contains coding sequences for a 6-histidine tag at the C-terminus of the expressed protein. The transmembrane (TM) domain of the B6R gene was deleted during subcloning so that the resulting vector would express only the extracellular domain of the protein. The plasmid was produced in Escherichia coli (E. coli) DH5a™ cells and extracted using a QIAGEN® EndoFree® Plasmid Maxi Kit. The pETMPOX/B5Ro?TM plasmid is reported to direct the expression of monkeypox B6R protein in inclusion bodies when transformed into an appropriate expression host cell such as E. coli BL21(DE3). The designation B5Ro is used to indicate that the B6R gene is the monkeypox ortholog of the vaccinia virus B5R gene.

Each vial contains approximately 20 to 60 ng of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pETMPOX/B5RoΔTM Containing the B6R Gene from Monkeypox Virus, Zaire 79, NR-3025."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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