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NR-41869   Expression Vector Containing the Heat Shock Protein 70 Gene Fragment from Biomphalaria glabrata, Recombinant in Escherichia coli
(Plasmid/Vectors)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Expression Vector Containing the Heat Shock Protein 70 Gene Fragment from Biomphalaria glabrata, Recombinant in Escherichia coli
Organism: Biomphalaria glabrata
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: M Tucker
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.

NR-41869 is an expression vector (pCR®2.1-TOPO®), containing a fragment of the heat shock protein (HSP) 70 gene from Biomphalaria glabrata (B. glabrata), transformed into Escherichia coli (E. coli), strain DH5a.

To create the insert, RNA was extracted from juvenile B. glabrata, strain NMRI, that were exposed to Schistosoma mansoni. First strand cDNA was synthesized with oligo-poly(dT)15. To construct the library, first strand cDNA was processed into all steps of suppression subtractive hybridization (SSH) using the Clontech® PCR-Select™ cDNA Subtraction Kit. PCR was performed using adaptor-specific primers for the HSP 70 gene fragment. The amplified product was cloned into pCR®2.1-TOPO® (Invitrogen™ Life Technologies™) cloning vector, and was transformed into E. coli, strain DH5a.

The expressed sequence tag (EST) of the HSP 70 gene fragment is available (GenBank: GH717081).

Each vial contains approximately 1 mL of E. coli, strain DH5a, in Luria Bertani (LB) broth containing 100 µg/mL of ampicillin supplemented with 15% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was provided by the NIAID Schistosomiasis Resource Center for distribution through BEI Resources, NIAID, NIH: Expression Vector Containing the Heat Shock Protein 70 Gene Fragment from Biomphalaria glabrata, Recombinant in Escherichia coli, NR-41869."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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