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NR-4194   Escherichia coli, Shuttle Vector pFNLTP1 for Gene Expression in Francisella Species and Escherichia coli
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Description: Shuttle Vector pFNLTP1 for Gene Expression in Francisella Species and Escherichia coli
Organism: Escherichia coli
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: TC Zahrt
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-4194 is a shuttle vector developed for use in a variety of genetic procedures in both Francisella species and Escherichia coli (E. coli), including characterization of virulence determinants. pFNLTP1 was obtained by spontaneous deletion of pTOPO/FNL10 and contains genes that confer resistance to kanamycin and ampicillin. The plasmid was transformed into E. coli DH5a cells (clone TCZ564) and extracted using a QIAGEN® Plasmid Mega Kit. pFNLTP1 has a molecular weight of 6872 base pairs.

The complete sequence of plasmid pFNLTP1 is available (GenBank: AY622904).

Note: Plasmid pFNLTP1 contains the gene required for kanamycin resistance. The recommended concentration of kanamycin in culture is 50 µg/mL.

Each vial contains approximately 1 µg of plasmid DNA in 10 mM Tris-HCl, pH ~ 8.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Shuttle Vector pFNLTP1 for Gene Expression in Francisella Species and Escherichia coli, NR-4194."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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