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NR-46128   Escherichia coli - Staphylococcus aureus Shuttle Vector pCN38, Recombinant in Staphylococcus aureus
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at no cost to registered researchers.
Description: Escherichia coli - Staphylococcus aureus Shuttle Vector pCN38, Recombinant in Staphylococcus aureus
Organism: Staphylococcus aureus
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: RP Novick
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-46128 is a culture of Staphylococcus aureus (S. aureus), strain 4220 (RN9591, NRS591) containing the Escherichia coli (E. coli)-staphylococcal shuttle vector pCN38. Vector pCN38 contains the E. coli ColE1 replication origin and the S. aureus pT181 cop-wt-repC replicon. Vector pCN38 was deposited as resistant to ampicillin and chloramphenicol in E. coli and resistant to chloramphenicol in S. aureus.

The complete sequence and vector map of pCN38 have been determined and are available on the Certificate of Analysis for lot 63341060. The BEI Resources vector sequence was deposited into GenBank as NR-46128 (GenBank: KR781477).

Each vial of NR-46128 contains approximately 0.5 mL of bacterial culture in Casitone-Yeast (CY) broth containing 0.1 M glycerol phosphate and 10 µg/mL chloramphenicol supplemented with 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Escherichia coli - Staphylococcus aureus Shuttle Vector pCN38, Recombinant in Staphylococcus aureus, NR-46128."
Additional Information: NARSA NRS591
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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