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NR-46158   Escherichia coli - Staphylococcus aureus Shuttle Vector pCN57, Recombinant in Staphylococcus aureus
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Escherichia coli - Staphylococcus aureus Shuttle Vector pCN57, Recombinant in Staphylococcus aureus
Organism: Staphylococcus aureus
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: RP Novick
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-46158 is a culture of Staphylococcus aureus (S. aureus), strain 4221 (RN9623, NRS623) containing the Escherichia coli (E. coli)-staphylococcal shuttle vector pCN57 (pNR-46158). Vector pCN57 contains the E. coli ColE1 replication origin, the S. aureus pT181 cop-wt-repC replicon, the Pblaz promoter and a promoterless ß-lactamase reporter gene, gfpmut2. Vector pCN57 was deposited as resistant to ampicillin and erythromycin in E. coli and resistant to erythromycin in S. aureus.

The complete sequence and vector map of pCN57 have been determined and are available on the Certificate of Analysis for lot 62782862. The complete BEI Resources vector sequence (pNR-46158) is available (GenBank: KP255997.)

Each vial of NR-46158 contains approximately 0.5 mL of bacterial culture in Casitone-Yeast (CY) broth containing 0.1 M glycerol phosphate and 10 µg/mL erythromycin supplemented with 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Escherichia coli - Staphylococcus aureus Shuttle Vector pCN57, Recombinant in Staphylococcus aureus, NR-46158."
Additional Information: NARSA NRS623
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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