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NR-49446  Plasmid Containing West Nile Virus, MAD78 Infectious Clone, pWNV-MAD-IC
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at no cost to registered researchers.
Description: West Nile virus
Organism: Plasmid Containing West Nile Virus, MAD78 Infectious Clone, pWNV-MAD-IC
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: BL Fredericksen

Quantity limit per order for this item is 1.  This item can be ordered twice a year.  Orders over this limit will be sent to NIAID for approval before shipment.

The entire West Nile virus (WNV) MAD78 (DakAnMg798) genome was cloned into the pWSK29 vector as described to generate plasmid pWNV-MAD-IC.  The plasmid was grown in Escherichia coli MDS™ 42 cells (Scarab Genomics) and extracted using a QIAGEN® Plasmid Plus Maxi Kit.

pWNV-MAD-IC contains a T7 bacteriophage promoter immediately upstream of the 5’ end of the viral genome.  Transfection of Vero cells with RNA transcribed in vitro from the linearized plasmid is reported to result in the production of infectious WNV with biological properties indistinguishable from the parental WNV-MAD78.

NR-49446 has been qualified for use in bacterial transformations.

Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid Containing West Nile Virus, MAD78 Infectious Clone, pWNV-MAD-IC, NR-49446.”
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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