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NR-50170   Plasmid pMRLB.7 Containing Gene Rv3875 (Protein ESAT-6) from Mycobacterium tuberculosis
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at no cost to registered researchers.
Description: Plasmid pMRLB.7 Containing Gene Rv3875 (Protein ESAT-6) from Mycobacterium tuberculosis
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: Temporarily Out of Stock (Please See Comments)
Store at: -80°C or colder
Contributor: NIH - TB Vaccine Testing and Research Materials Contract
Quantity limit for this item is 2 per year. Orders over this limit will be sent to NIAID for approval before shipment.

This item is currently in our production queue. Please allow ample time for distribution lots to be made available.

NR-50170 is a recombinant expression vector containing Mycobacterium tuberculosis gene Rv3875, which encodes the early secretory antigenic target ESAT-6, also known as esxA. Gene Rv3875 was amplified by PCR and cloned into pET23b for expression in Escherichia coli. The gene was cloned without a signal sequence and with nucleotides coding for the amino acids phenylalanine, alanine, leucine and glutamic acid (FALE) prior to the histidine tag.  These nucleotides increase plasmid stability and promote solubility upon transformation and expression. The expressed protein has an observed molecular weight of 10 kDa. 

Note: Plasmid pMRLB.7 contains the gene required for ampicillin (Ap) resistance. The recommended concentration of Ap in culture is 100 µg/mL.

Each vial contains approximately 50 µl of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA buffer, pH 8).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pMRLB.7 Containing Gene Rv3875 (Protein ESAT-6) from Mycobacterium tuberculosis, NR-50170."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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