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NR-52394   Vector pCAGGS Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike Glycoprotein Gene (soluble, stabilized)
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Description: Vector pCAGGS Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike Glycoprotein Gene (soluble, stabilized)
Organism: SARS-Related Coronavirus 2
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: F Krammer, F Amanat, S Strohmeier
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The vector for the spike (S) glycoprotein gene from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenBank: MN908947) was designed for expression of a soluble S glycoprotein (residues 1 to 1213) with a polybasic cleavage site deletion (RRAR to A; residues 682 to 685) and stabilizing mutations (K986P and V987P, wild type numbering) with a C-terminal thrombin cleavage site, T4 foldon trimerization domain and hexa-histidine tag. The S sequence was codon optimized for mammalian expression and subcloned into the pCAGGS mammalian expression vector. The resulting size of the plasmid is approximately 8530 base pairs. The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in Escherichia coli (E. coli) and extracted.

The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.

Note: For a detailed protocol and list of related items, see

Each vial contains 0.6 µg of plasmid DNA in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression.
Citations: Acknowledgment for publications should read ““The following reagent was produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Vector pCAGGS Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike Glycoprotein Gene (soluble, stabilized), NR-52394.”
Restrictions On Use:
Registrants interested in commercializing this product must contact Mount Sinai for a license (Frenz, Christopher
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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