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NR-9343   Plasmid Containing a Segment of the E9L Gene from Vaccinia Virus, New York City Board of Health, Linearized
(Plasmid/Vectors)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Plasmid Containing a Segment of the E9L Gene from Vaccinia Virus, New York City Board of Health, Linearized
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: NIH Biodefense and Emerging Infections Research Resources Repository
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

A segment of the E9L gene from vaccinia virus, New York City Board of Health (BEI Resources NR-54) was amplified from extracted DNA and cloned into a commercial vector. The plasmid was produced in Escherichia coli DH5α™-T1R cells, extracted using a QIAGEN® Plasmid Maxi Kit and linearized with HindIII.

NR-9343 has been qualified for use as a standard for real-time quantitative PCR. A Pan-Orthopox Virus E9L Gene-Specific Quantitative PCR Assay Detection Kit containing forward and reverse primers, probe and NR-9343 is available as BEI Resources NR-9350.

Each vial contains approximately 45 ng of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid Containing a Segment of the E9L Gene from Vaccinia Virus, New York City Board of Health, Linearized, NR-9343."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-54
Vaccinia virus New York City Board of Health (Wyeth, Calf Adapted)
NR-9350
Orthopoxvirus Pan-Orthopox Virus E9L Gene-Specific Quantitative PCR Assay Detection Kit
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