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MRA-220  Plasmodium falciparum, dhfr-M5 Reverse Primer
(Primers and Probes)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: dhfr-M5 Reverse Primer
Organism: Plasmodium falciparum
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: -20°C
Contributor: MR4
MRA-220, Plasmodium falciparum, dhfr-M5 Reverse Primer, is no longer available.

Primer dhfr-M5 is a twenty-one nucleotide reverse primer designed for detection of the dihydrofolate reductase gene (dhfr) of Plasmodium falciparum when paired with its partner primer (MRA-219). The sequence of the dhfr-M5 21-mer is 5'-AGTATATACATCGCTAACAGA-3'.

Each vial contains between 10 µg and 60 µg of freeze-dried primer. Dissolving the contents of one vial (2.5 nmole) in 100 µL of distilled water and mixing with its partner primer (total volume of 200 µL), MRA-220 can be used for 200 PCR reactions (each reaction uses 1 µL in a 25 µL volume) at 500 nM. If used at 250 nM, 400 PCR reactions can be completed. If used at 250 nM, 400 reactions can be run. If three or four primers are used, the above volumes would be 1.5 and 2.0 µL, respectively, per reaction.

Duraisingh, M. T., J. Curtis and D. C. Warhurst. "Plasmodium falciparum: Detection of Polymorphisms in the Dihydrofolate Reductase and Dihydropteroate Synthetase Genes by PCR and Restriction Digestion." Exp. Parasitol. 89 (1998): 1-8. PubMed: 9603482.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium falciparum, dhfr-M5 Reverse Primer, MRA-220, contributed by Malaria Research and Reference Reagent Resource Center (MR4)."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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