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Plasmodium falciparum (P. falciparum) gametocyte-specific (Pfg27) protein
is expressed early in gametocytes during sexual differentiation. Mouse polyclonal antibodies against Pfg27 are specific for stage I to V gametocytes of P. falciparum.
recombinant form of the Pfg27 protein containing an N-terminal maltose
binding protein (MBP) and a C-terminal histidine tag was expressed in Escherichia coli. After initial purification with Dextrin
Sepharose and Sephacryl 200, the MBP fragment was removed with Factor Xa and
the resulting His6-tagged protein was purified by nickel affinity
chromatography. MRA-1274 has a theoretical
molecular weight of approximately 27 kilodaltons.
vial contains approximately 250 µg of purified recombinant Pfg27 protein in 20
mM Tris-HCl (pH 8) with 500 mM NaCl.
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biological materials provided by BEI Resources, please select this link: Compliance Requirements.
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