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NR-12193   Intimin Alpha C-Terminal Extracellular Domain from Escherichia coli O127:H6 with N-Terminal Histidine Tag, Expressed in Escherichia coli
(Proteins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Intimin Alpha C-Terminal Extracellular Domain from Escherichia coli O127:H6 with N-Terminal Histidine Tag, Expressed in Escherichia coli
Organism: Escherichia coli
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: AD O'Brien, JF Sinclair
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-12193 is a recombinant form of the C-terminal extracellular domain of the Escherichia coli (E. coli) O127:H6 outer membrane protein intimin alpha. Intimin, encoded by the eaeA gene, is an adhesin that is required for adherence of enterohemorrhagic E. coli (EHEC) to human epithelial cells and colonization of experimentally infected animals. The protein sequence includes an N-terminal hexa-histidine tag and amino acid residues of intimin alpha from EPEC O127:H6, strain E2348/69. The recombinant protein was expressed in E. coli and purified by nickel affinity chromatography. NR-12193 has a theoretical molecular weight of 42,717 daltons.

Each vial contains approximately 500 µg of NR-12193 in PBS.

NR-12193 reacts with rabbit polyclonal antibody to E. coli intimin gamma (BEI Resources NR-12194) as shown by western blot analysis. Note: Antiserum developed against intimin gamma subtype also reacts with other intimin subtypes.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Intimin Alpha C-Terminal Extracellular Domain from Escherichia coli O127:H6 with N-Terminal Histidine Tag, Expressed in Escherichia coli, NR-12193."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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