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NR-19236   Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, B/Florida/4/2006, Recombinant from Baculovirus
(Proteins)
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at no cost to registered researchers.
Description: Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, B/Florida/4/2006, Recombinant from Baculovirus
Organism: Influenza B virus
Biosafety Level: 1
Availability Status: Temporarily Out of Stock (Please See Comments)
Store at: 2°C to 8°C
Contributor: BEI Resources
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.

This item is currently in our production queue. Please allow ample time for distribution lots to be made available.


The neuraminidase (NA) protein from influenza B virus, B/Florida/4/2006 containing an N-terminal histidine tag was produced in Sf9 (Invitrogen™ 11496-015) insect cells using a baculovirus expression vector system and was purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal eight-histidine tag followed by a 43 amino acid tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site, as described for the 1918 pandemic virus. The predicted protein sequence is shown in Table 1 on the product sheet. The full-length NA precursor protein is 466 residues (GenPept: ABU50667).

NR-19236 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-a-D-N-acetylneuraminic acid (4-MUNANA).

Each vial contains approximately 50 µg of purified recombinant NA protein in 10 mM sodium phosphate pH 7.4 with 500 mM sodium chloride.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, B/Florida/4/2006, Recombinant from Baculovirus, NR-19236."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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