Contact Us   Having technical issues or questions regarding this product?
Please Contact Us.
BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-32875  Yersinia pestis, LcrV Protein, Recombinant from Escherichia coli
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: LcrV Protein, Recombinant from Escherichia coli
Organism: Yersinia pestis
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: BEI Resources
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Yersinia pestis (Y. pestis), the causative agent of the plague, secretes massive amounts of LcrV (low-calcium-response V or V antigen) during infection. Mutations that abrogate the expression of LcrV render Y. pestis avirulent. LcrV is a multifunctional protein that is central to the activity of the type III secretion apparatus of Y. pestis. It has no known catalytic function, and its biological activity is dependent on interactions with other proteins. Injection of LcrV into animals stimulates humoral responses that offer protection against plague infection. The amino acid sequence for LcrV from Y. pestis has been reported by 2 groups in the NCBI protein database (AAC62574 and AAC69799). The crystal structure for LcrV from Y. pestis has been solved at 2.17 Å resolution (PDB: 1R6F).

Recombinant LcrV protein (Y. pestis, strain KIM5) was expressed in Escherichia coli BL21 (DE3) cells as described. The N-terminal deca-histidine tagged protein was purified via nickel-NTA and gel filtration chromatography. Cleavage with Factor Xa resulted in a protein with one extra histidine at the amino acid terminus. The protein preparation was treated with Triton X-114 to reduce endotoxin contamination. NR-32875 has the same amino acid sequence as NR-3832 and was expressed and purified using similar methodology but was produced at BEI Resources, not the University of Chicago.

Each vial contains approximately 1 mg of recombinant LcrV protein in PBS, pH 7.4.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Yersinia pestis LcrV Protein, Recombinant from Escherichia coli, NR-32875."
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Return to Top

Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

Back to My Search