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NR-4284  SARS coronavirus, Envelope (E) Protein, Recombinant from Escherichia coli
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Description: Envelope (E) Protein, Recombinant from Escherichia coli
Organism: SARS coronavirus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C
Contributor: NIH Biodefense and Emerging Infections Research Resources Repository
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The SARS-CoV E-SUMO fusion protein containing an N-terminal histidine tag was expressed in Escherichia coli and purified by nickel affinity, ion exchange and size exclusion chromatography. After the fusion was cleaved by the SUMO protease, the SUMO tag and the SUMO protease (both histidine-tagged) were subtracted from the SARS CoV E protein by nickel affinity chromatography. The SARS CoV E protein was dialyzed against 10 mM ammonium bicarbonate and aliquoted into 10 mM sodium carbonate and 10 mM sodium phosphate, vacuum dried and stored at 20°C.

Each vial contains approximately 0.1 mg of E protein (determined by Bradford assay with BSA as a standard), 20 micrograms sodium carbonate and 31 micrograms sodium phosphate.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-CoV Envelope (E) Protein, Recombinant from Escherichia coli, NR-4284."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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