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NR-52308   Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1, Recombinant from Baculovirus
(Proteins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1, Recombinant from Baculovirus
Organism: SARS-Related Coronavirus 2
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: F Krammer, F Amanat, S Strohmeier
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced in SF9 insect cells using a baculovirus expression system and purified by nickel affinity chromatography. NR-52308 lacks the signal sequence and contains 1196 residues (ectodomain) of the SARS-CoV-2 spike glycoprotein; the recombinant protein was modified to remove the polybasic S1/S2 cleavage site (RRAR to A; residues 682 to 685), stabilized with a pair of mutations (K986P and V987P, wild type numbering) and includes a thrombin cleavage site, T4 foldon trimerization domain and C-terminal hexa-histidine tag. NR-52308 has a theoretical molecular weight of 137,600 daltons.

Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/

Each vial of lot 70034410 contains 250 µL of NR-52308 in phosphate buffered saline (PBS).

Additional information and tools are available at ViPR (Virus Pathogen Resource).
Citations: Acknowledgment for publications should read “The following reagent was produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1, Recombinant from Baculovirus, NR-52308.”
Restrictions On Use:
Registrants interested in commercializing this product must contact Mount Sinai for a license (Frenz, Christopher christopher.frenz@mssm.edu).
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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RELATED PRODUCTS
NR-52396
Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1, Recombinant from Baculovirus
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