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NR-545  Vaccinia virus, (WR) A33R Protein with C-terminal Histidine Tag, Recombinant from baculovirus
(Proteins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: (WR) A33R Protein with C-terminal Histidine Tag, Recombinant from baculovirus
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: GH Cohen, RJ Eisenberg
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-545 is a recombinant form of the A33R membrane glycoprotein [A33Rt; residues 58 to 185, C-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus. The full length A33R protein is 185 residues. NR-545 was produced in Sf9 insect cells using a baculovirus expression system and was purified using ammonium sulfate precipitation and nickel affinity chromatography. NR-545 was demonstrated to be functionally active based on its reactivity with human polyclonal anti-vaccinia virus immune globulin (VIG) and a monoclonal antibody to A33R (BEI Resources NR-777).

Each vial contains approximately 0.5 mg of NR-545 in 50 mM borate buffer (pH 8) containing 100 mM NaCl.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus (WR) A33R Protein with C-terminal Histidine Tag, Recombinant from Baculovirus, NR-545."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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