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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-546  Vaccinia virus, (WR) B5R Protein with N-terminal Histidine Tag, Recombinant from Baculovirus
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: (WR) B5R Protein with N-terminal Histidine Tag, Recombinant from Baculovirus
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: GH Cohen, RJ Eisenberg
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-546 is a recombinant form of the B5R membrane glycoprotein [B5R(275t); residues 20 to 275 comprising the ectodomain, N-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus. The full length B5R protein is 317 residues. NR-546 was produced in Sf9 insect cells using a baculovirus expression system and was purified using ammonium sulfate precipitation and nickel affinity chromatography. NR-546 was demonstrated to be functionally active based on its reactivity with human polyclonal anti-vaccinia virus immune globulin (VIG) and monoclonal antibodies to B5R (BEI Resources NR-422 to NR-424, NR-426 to NR-431, NR-551 to NR-556, and NR-559 to NR-562).

Each vial contains approximately 0.5 mg of NR-546 in 50 mM borate buffer (pH 8) containing 100 mM NaCl.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus (WR) B5R Protein with N-terminal Histidine Tag, Recombinant from baculovirus, NR-546."
Publications Citing
this Reagent:
Garber, D. A., et al. "Deletion of Specific Immune Modulatory Genes from Modified Vaccinia Ankara-Based HIV Vaccines Engenders Improved Immunogenicity in Rhesus Macaques." J. Virol. 86 (2012): 12605-12615. PubMed: 22973033.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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