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NR-654   H9 HA Protein from Influenza Virus, A/Hong Kong/1073/1999 (H9N2), Recombinant from Baculovirus
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Description: H9 HA Protein from Influenza Virus, A/Hong Kong/1073/1999 (H9N2), Recombinant from Baculovirus
Organism: Influenza A virus
Biosafety Level: 1
Availability Status: In Stock
Store at: 4°C
Contributor: NIH - Influenza Pandemic Preparedness in Asia Program
Quantity Limit per Order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Recombinant H9 hemagglutinin (HA) protein from influenza virus A/Hong Kong/1073/1999 (H9N2) was produced in Sf9 insect cells using a baculovirus expression vector system. Recombinant H9 HA protein was purified using conventional chromatographic techniques. NR-654 is biologically active in a hemagglutination assay with 0.5% chicken red blood cells. NR-654 is specific to H9 HA subtype of influenza virus as determined in serological hemagglutination inhibition (HI) assays. Within the H9 HA subtype, NR-654 reacts in HI and ELISA assays with reference antisera of the G1 sublineage, but not with reference antisera of the G9 sublineage.

Each vial contains approximately 0.25 mL (46.8 micrograms) of purified recombinant H9 HA protein in 10 mM sodium phosphate (pH 7.0), 150 mM sodium chloride, and 0.01% Tween-20.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: H9 Hemagglutinin (HA) Protein from Influenza Virus, A/Hong Kong/1073/1999 (H9N2), Recombinant from baculovirus, NR-654."
Publications Citing
this Reagent:
McCullough, C., et al. "Characterization of Influenza Hemagglutinin Interactions with Receptor by NMR." PLoS One. 7 (2012): e33958. PubMed: 22815674.

Thomson, C. A., et al. "Pandemic H1N1 Influenza Infection and Vaccination in Humans Induces Cross-Protective Antibodies that Target the Hemagglutinin Stem." Front. Immunol.. 3 (2012): doi: 10.3389/fimmu 2012.00087. PubMed: 22586427.

Wang, T. T., et al. "Vaccination with a Synthetic Peptide from the Influenza Virus Hemagglutinin Provides Protection against Distinct Viral Subtypes." Proc.Natl. Acad. Sci. USA 107 (2010): 18979-18984. PubMed: 20956293

Wongphatcharachai, M., et al. "Neutralizing DNA Aptamers Against Swine Influenza H3N2 Viruses." J. Clin. Microbiol. 51 (2013): 46-54. PubMed: 23077124
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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