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NR-686  SARS coronavirus, Spike (S) Protein with C-terminal Histidine Tag, Recombinant from Baculovirus
(Proteins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Spike (S) Protein with C-terminal Histidine Tag, Recombinant from Baculovirus
Organism: SARS coronavirus
Biosafety Level: 1
Availability Status: In Stock
Store at: 4°C
Contributor: NIH Biodefense and Emerging Infections Research Resources Repository
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-686 is a full-length, glycosylated recombinant form of the SARS-CoV spike (S) external envelope glycoprotein and contains a C-terminal histidine tag. A baculovirus signal sequence, MPLYKLLNVLWLVAVSNA, was added to the N-terminal end of the protein to enhance expression. The SARS-CoV spike (S) protein was produced in Sf9 insect cells using a baculovirus expression vector system and was purified using nickel affinity chromatography. The baculovirus signal sequence is efficiently removed during translocation of the protein across the membrane. The resulting protein, NR-686, has a molecular weight of approximately 160,000 daltons. The purified protein was demonstrated to be functionally active based on its ability to bind to a recombinant form of its functional receptor, angiotensin I converting enzyme-2.

Each vial contains approximately 0.5 mg of NR-686 in 20 mM Tris-HCl (pH 8.0), 150 mM NaCl and 0.005% Tween-20.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-CoV Spike (S) Protein with C-terminal Histidine Tag, Recombinant from Baculovirus, NR-686."
Publications Citing
this Reagent:
Andonov, A. "Neutralizing Epitopes of the SARS-CoV S-Protein Cluster Independent of Repertoire, Antigen Structure or mAb Technology." Mabs. 2 (2010):53-66. PubMed: 20168090.

Sheahan, T., et al. "Successful Vaccination Strategies that Protect Aged Mice from Lethal Challenge from Influenza Virus and Heterologous Severe Acute Respiratory Syndrome Coronavirus." J. Virol. 85 (2011): 217-230. PubMed: 20980507.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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