Contact Us   Having technical issues or questions regarding this product?
Please Contact Us.
BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-700  SARS coronavirus, 3CL Protease, Recombinant from E. coli
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: 3CL Protease, Recombinant from E. coli
Organism: SARS coronavirus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: NIH Biodefense and Emerging Infections Research Resources Repository
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-700 was expressed and purified using a novel SUMO fusion system. An N-terminal histidine-tagged SUMO-3CL protease fusion was expressed in E. coli and purified by nickel affinity chromatography. After the fusion was cleaved by the SUMO protease, the SUMO tag and the SUMO protease (both histidine-tagged) were subtracted from the 3CL protease by nickel affinity chromatography. The 3CL protease was further purified by anion exchange chromatography, dialyzed against 10 mM ammonium bicarbonate, aliquoted and lyophilized. NR-700 has a molecular weight of approximately 33,800 daltons. Using Western blot analysis, NR-700 reacted with rabbit polyclonal sera prepared against the SUMO 3CL protease fusion, but did not react with rabbit polyclonal sera prepared against a SUMO nucleocapsid fusion.

Each vial contains 1.0 mg of NR-700 lyophilized in 10 mM ammonium bicarbonate.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-CoV 3CL Protease, Recombinant from E. coli, NR-700."
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Return to Top

Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

Back to My Search