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NR-878  SARS coronavirus, Membrane (M) Protein with C-terminal Histidine Tag, Recombinant from E. coli
(Proteins)
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Description: Membrane (M) Protein with C-terminal Histidine Tag, Recombinant from E. coli
Organism: SARS coronavirus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C
Contributor: NIH Biodefense and Emerging Infections Research Resources Repository
Comments:
Quantity Limit per Order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The SARS-CoV membrane (M) glycoprotein is the most abundant structural protein of the virus and plays a key role in virion morphogenesis and release. During virion assembly, it is localized at specialized intracellular membrane structures. Interactions between the M protein, the envelope (E) protein and nucleocapsids [viral RNA and the nucleocapsid (N) protein] result in budding through the membrane. The spike (S) protein is incorporated into the viral envelope by interaction with the M protein and mature virions are released from smooth vesicles.

The M protein is highly hydrophobic with three membrane spanning domains in the amino-terminal half of the protein. A highly conserved amino acid sequence, SMWSFNPE, immediately follows the third hydrophobic domain. The M protein has an NGT near its amino terminus, suggesting that it is N-glycosylated at position 4.

The SARS-CoV M protein containing a C-terminal histidine tag (NR-878) was expressed in E. coli and purified by nickel affinity, cation exchange and size exclusion chromatography. NR-878 was extensively dialyzed against 10 mM ammonium bicarbonate and 0.3% lauryl sarcosine, aliquoted and lyophilized. NR-878 has a molecular weight of approximately 26,700 daltons.

Each vial contains approximately 1.0 mg of M protein (determined by Bradford assay) and 3 mg of lauryl sarcosine lyophilized from 1 mL of 10 mM ammonium bicarbonate and 0.3% lauryl sarcosine.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-CoV Membrane (M) Protein with C-terminal Histidine Tag, Recombinant from E. coli, NR-878."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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