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NR-9577 Bacillus anthracis, Collagen-like Protein BclA (Locus_Tag: BA_1222) with N-terminal Histidine Tag, Recombinant from Escherichia coli
(Proteins)

Price: All BEI Resources products are provided
at no cost to registered researchers.

Description:

Collagen-like Protein BclA (Locus_Tag: BA_1222) with N-terminal Histidine Tag, Recombinant from Escherichia coli

Organism:

Bacillus anthracis

Biosafety Level:

Availability Status:

In Stock

Store at:

-80°C or colder

Contributor:

AD O'Brien, JF Sinclair

Comments:

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-9577 is a recombinant form of the Bacillus anthracis (B. anthracis) collagen-like protein BclA (locus_tag: BA_1222), a major component of the exosporium. The amino acid sequence includes 1) an N-terminal hexa-histidine tag, 2) a thrombin cleavage site and 3) the complete coding sequence (amino acid residues 1 to 400) of BclA from the Sterne strain (GenPept: AAT53453). The recombinant protein was expressed in Escherichia coli and purified by nickel affinity chromatography. NR-9577 has a theoretical molecular weight of approximately 39 kilodaltons. The structure of BclA has been solved (PDB: 1WCK; 2R6Q).

Each vial contains approximately 80 to 120 µg of NR-9577 in PBS containing 2 M urea.

NR-9577 reacts with rabbit polyclonal antibody to B. anthracis BclA (BEI Resources NR-9578) as shown by western blot analysis.

Citations:

Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Bacillus anthracis Collagen-like Protein BclA (Locus_Tag: BA_1222) with N-terminal Histidine Tag, Recombinant from Escherichia coli, NR-9577."

Publications Citing this Reagent:

Goldman, E. R., et al. "Negative Tail Fusions Can Improve Ruggedness of Single Domain Antibodies." Protein Expr. Purif. 95 (2014): 226-232. PubMed: 24440507.

Walper, S. A., et al. "Selection and Characterization of Single Domain Antibodies Specific for Bacillus anthracis Spore Proteins." Antibodies 2 (2013): 152-167.

Walper, S. A., et al. "Thermostable Single Domain Antibody-Maltose Binding Protein Fusion for Bacillus anthracis Spore Protein BclA Detection." Anal. Biochem. 447 (2014): 64-73. PubMed: 24184358

Restrictions On Use:

This material is distributed for internal research, noncommercial purposes only. This material, its product or its derivatives may not be distributed to third parties. Except as performed under a U.S. Government contract, individuals contemplating commercial use of the material, its products or its derivatives must contact the contributor to negotiate a license. U.S. Government contractors may need a license before first commercial sale.

Product Name:

Collagen-like Protein BclA (Locus_Tag: BA_1222) with N-terminal Histidine Tag, Recombinant from Escherichia coli

Disclaimers:

You are authorized to use this product for research use only. It is not intended for human use.

Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC^® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC^® nor the U.S. Government warrants that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC^® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC^®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products.

Citation:

Biosafety Level:

1 Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.

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Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

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Product Highlights for NR-9577

Product Sheet Information for NR-9577 Collagen-like Protein BclA (Locus_Tag: BA_1222) with N-terminal Histidine Tag, Recombinant from Escherichia coli, Bacillus anthracis, For research use only. Not for use in humans.

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