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MRA-1300  Anopheles coluzzii, AgORCO-QF2, Eggs
(Vectors)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Designations: AgORCO-QF2, Eggs
Organism: Anopheles coluzzii
Biosafety Level: 1
Availability Status: In Stock
Store at: 24°C to 30°C, 50 to 80% relative humidity
Contributor: CJ Potter
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Anopheles coluzzii (An. coluzzii) colony originated in Ngousso, Cameroon.

An. coluzzii, strain AgORCO-QF2 is a new transgenic strain utilizing the binary expression system QF-QUAS (Q-system). The strain was produced using a piggyBac vector containing DsRed under control of the synthetic eye promoter 3xP3. QF2 is a genetically modified transcriptional activator that is derived from the qa gene cluster of the fungus Neurospora crassa that works well in Drosophila and Anopheles insects. This strain is a driver construct that expresses a 9 Kb enhancer + promoter region from Orco, the odorant receptor coreceptor expressed in An. coluzzii olfactory neurons. This transgenic line can be used in combination with QUAS-geneX effector constructs to express geneX in olfactory neurons.

Note: MRA-1300 is a Q-system driver line, and must be crossed with a Q-system effector line for tissue-specific gene expression. MRA-1300 can be crossed with MRA-1301.

MRA-1300 contains a suitable number of eggs to establish a stock. Eggs are provided on damp filter paper and should be hatched immediately upon receipt.
Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Anopheles coluzzii, Strain AgORCO-QF2, Eggs, MRA-1300, contributed by Christopher J. Potter.”
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
MRA-1301
Anopheles coluzzii QUAS-CD8:GFP, Eggs
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