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Product Name:
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G3, Bulk Frozen
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA
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Taxonomy:
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Protozoa Classification: Culicidae, Anopheles Species: Anopheles gambiae Strain: G3
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Additional Information:
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Strain G3 has a 2La/+, 2r+/+, TEP1 s/s genotype.2,3,4 G3 is a mongrel stock that has not been exhaustively defined to distinguish it from other 'wild' An. gambiae stocks.1 It is reported as Savanna rDNA form (predominantly) and dieldrin-susceptible, and is distributed 'as is' with accompanying authentication information (wild eye color, polymorphic at collarless).1,2,3,4
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Material Provided:
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Each vial of MRA-132B contains approximately 100 to 200 An. gambiae frozen male and female mosquitos, which were preserved in liquid nitrogen (quick-frozen) while alive.
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Packing/Storage:
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MRA-132B is prepared by and shipped from CDC, Atlanta, Georgia, USA. The product is provided frozen and should be stored at -80°C or colder immediately upon arrival.
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Functional Activity:
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Applications: MRA-132B is suitable for DNA and RNA isolation, protein extraction, etc.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Collins, W. E., Personal Communication.
2. Beard, C. B., et al. “Eye Pigments in Wild-Type and Eye-Color Mutant Strains of the African Malaria Vector Anopheles gambiae.” J. Hered. 86 (1995): 375-380. PubMed: 7560874.
3. For details on authentication methods used to confirm the identity of this G3 stock, please refer to beiresources.org/portals/2/MR4.
4. Scott, J. A., W. G. Brogdon and F. H. Collins. “Identification of Single Specimens of the Anopheles gambiae Complex by the Polymerase Chain Reaction.” Am. J. Trop. Med. Hyg. 49 (1993): 520-529. PubMed: 8214283.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Anopheles gambiae, Strain G3, Bulk Frozen, MRA-132B, contributed by Centers for Disease Control and Prevention (CDC)."
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Biosafety Level:
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1
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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