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Product Name:
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Eggs
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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Darold P. Batzer, Professor, and Elmer W. Gray, Assistant Project Director, Black Fly Research and Resources Center (BFR2), Department of Entomology, The University of Georgia, Athens, Georgia, USA
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Taxonomy:
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Classification: Simuliidae, Simulium
Species: Simulium vittatum sensu stricto (common name: black fly)
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Material Provided:
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NR-53890 contains a suitable number of eggs to establish a stock. Eggs are provided on wet, backlit fabric strips, in sealed Petri dishes including a paper towel moistened with deionized water. The product is shipped on blue ice to keep the eggs cool during shipping.
Note: Live S. vittatum is also available in larval (NR-53891), pupal (NR-53892) or adult stages (NR-53893).
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Packing/Storage:
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NR-53890 is prepared and shipped by the University of Georgia Black Fly Research and Resource Center. Upon arrival, the eggs should be immersed in 20°C to 22°C water to initiate the hatching process. Larvae will begin emerging in 3 to 4 days of receipt. Eggs may be stored in refrigeration (4°C to 8°C) for a short period, but viability will decrease if stored for an extended duration.
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Growth Conditions:
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Standard S. vittatum rearing procedures are recommended.5,6 See Appendix for details on handling S. vittatum eggs.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Brockhouse, C. L. and P. H. Adler. “Cytogenetics of Laboratory Colonies of Simulium vittatum Cytospecies IS-7 (Diptera: Simuliidae).” J. Med. Entomol. 39 (2002): 293-297. PubMed: 11931029.
2. Reis, J. L., Jr., et al. “Lesion Development and Replication Kinetics During Early Infection in Cattle Inoculated with Vesicular Stomatitis New Jersey Virus via Scarification and Black Fly (Simulium vittatum) Bite.” Vet. Pathol. 48 (2011): 547-557. PubMed: 20858740.
3. Adler, P. H., D. C. Currie and D. M. Wood. The Blackflies (Simuliidae) of North America. (2004) New York, New York: ROM Publication in Sciences.
4. Lehmann, T., M. S. Cupp and E. W. Cupp. “Analysis of Migration Success of Onchocerca lienalis Microfilariae in the Haemocoel of Simulium vittatum.” J. Helminthol. 69 (1995): 47-52. PubMed: 7622790.
5. Gray, E. W. and R. Noblet. “Black Fly Rearing and Use in Laboratory Information: Bioassays.” Rearing Animal and Plant Pathogen Vectors. (2014) Maramorosch, K., and F. Mahmood (Eds.) Boca Raton: CRC Press.
6. Bernardo, M. J., E. W. Cupp and A. E. Kiszewski. “Rearing Black Flies (Diptera: Simuliidae) in the Laboratory: Colonization and Life Table Statistics for Simulium vittatum.” Ann. Entomol. Soc. Am. 79 (1986): 610-621.
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Citation:
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Acknowledgment for publications should read “The Simulium vittatum used in this work were produced with the support of NIH Task Order C-08, Contract No. HHSN2722017000351, Task Order No. 75N93020F00002 and obtained through BEI Resources, NIAID, NIH: Simulium vittatum, Eggs, NR-53890.”
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Biosafety Level:
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1
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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