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Product Name:
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S5-A14a
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: unclassified Clostridiales Family XIII Incertae Sedis
Note: The label on the vial for lot 70012281 is incorrect; the correct nomenclature is Family XIII Incertae Sedis
Order: Clostridiales
Strain: S5-A14a
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Additional Information:
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Clostridiales bacteria are generally Gram-positive, rod-shaped, obligate anaerobes that are ubiquitous in virtually all anoxic habitats where organic compounds are found, especially soils, aquatic sediments and the intestinal tracts of animals and humans. Bacteria of the order Clostridiales have a Gram-positive cell wall but may stain Gram-variable or Gram-negative. 2,3,4,5 Most species have the ability to form spores 5,6 and a few are pathogenic, producing very potent biological toxins known to affect humans.7
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy Yeast Extract broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-1098 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Tryptic Soy Yeast Extract broth or equivalent
Tryptic Soy agar with 5% defibrinated sheep blood or Tryptic Soy Yeast Extract agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Anaerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 4 to 7 days
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Izard, J., Personal Communication.
2. Lawson, P. A., et al. "Anaerobes: A Piece in the Puzzle for Alternative Biofuels." Anaerobe 17 (2011): 206-210. PubMed: 21699990.
3. Ueki, A., et al. “Aminipila butyrica gen. nov., sp. nov., a Strictly Anaerobic, Arginine-Decomposing Bacterium Isolated from a Methanogenic Reactor of Cattle Waste.” Int. J. Syst. Evol. Microbiol. 68 (2018): 443-448. PubMed: 29235979.
4. Bernard, K., et al. “Characterization of Isolates of Eisenbergiella tayi, a Strictly Anaerobic Gram-Stain Variable Bacillus Recovered from Human Clinical Materials in Canada.” Anaerobe 44 (2017): 128- 132. PubMed: 28279858.
5. Mallozzi, M., V. K. Viswanathan and G. Vedantam. “Spore-forming Bacilli and Clostridia in Human Disease.” Future Microbiol. 5 (2010): 1109-1123. PubMed: 20632809.
6. Paredes-Sabja, D., P. Setlow and M. R. Sarker. “Germination of Spores of Bacillales and Clostridiales Species: Mechanisms and Proteins Involved.” Trends Microbiol. 19 (2011): 85-94. PubMed: 21112786.
7. Popoff, M. R. and P. Bouvet. “Clostridial Toxins.” Future Microbiol. 4 (2009): 1021-1064. PubMed: 19824793.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Clostridiales bacterium, Strain S5-A14a, HM-1098.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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