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Product Name:
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JCP7275
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Bifidobacteriaceae, Gardnerella
Species: Gardnerella vaginalis
Strain: JCP7275
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Additional Information:
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Gardnerella species are pleomorphic, non-motile, facultatively anaerobic bacteria, with some strains described as obligately anaerobic.3,4 Gardnerella are often described as Gram-negative to Gram-variable, however, analysis of the ultrastructure and composition of the cell wall determined that Gardnerella have a thin, atypical Gram-positive cell wall consisting of a lower peptidoglycan content that tends to stain Gram-negative.5,6 Although Gardnerella is commonly found in the vaginal microbiota of healthy individuals, it is one of the predominant organisms of the vaginal cavity in women with bacterial vaginosis.7,8 Originally consisting of a single species, Gardnerella vaginalis, the genus was emended to include 13 genomic species, with several later classified as the novel species greenwoodii, pickettii, piotii and swidsinskii.9,10
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in NYC III broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-1105 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
NYC III broth or equivalent
Chocolate agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Anaerobic or aerobic with 5% CO2
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 to 2 days.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Lewis, A., Personal Communication.
2. Lewis, W. G., et al. “Degradation, Foraging, and Depletion of Mucus Sialoglycans by the Vagina-Adapted Actinobacterium Gardnerella vaginalis.” J. Biol. Chem. 288 (2013): 12067-12079. PubMed: 23479734.
3. Greenwood, J. R. and M. J. Pickett. “Transfer of Haemophilus vaginalis Gardner and Dukes to a New Genus, Gardnerella: G. vaginalis (Gardner and Dukes) comb. nov.” Int. J. Syst. Bacteriol. 30 (1980): 170-178.
4. Malone, B. H., et al. “Obligately Anaerobic Strains of Corynebacterium vaginale (Haemophilus vaginalis).” J. Clin. Microbiol. 2 (1975): 272-275. PubMed: 1080766.
5. Catlin, B. W. “Gardnerella vaginalis: Characteristics, Clinical Considerations and Controversies.” Clin. Microbiol. Rev. 5 (1992): 213-237. PubMed: 1498765.
6. Harper, J. J. and G. H. G. Davis. “Cell Wall Analysis of Gardnerella vaginalis (Haemophilus vaginalis).” Int. J. Syst. Bacteriol. 32 (1982): 48-50.
7. Aroutcheva, A. A., et al. “Gardnerella vaginalis Isolated from Patients with Bacterial Vaginosis and from Patients with Healthy Vaginal Ecosystems.” Clin. Infect. Dis. 33 (2001): 1022-1027. PubMed: 11528575.
8. Yeoman, C. J., et al. “Comparative Genomics of Gardnerella vaginalis Strains Reveals Substantial Differences in Metabolic and Virulence Potential.” PLoS One 5 (2010): e12411. PubMed: 20865041.
9. Vaneechoutte, M., et al. “Emended Description of Gardnerella vaginalis and Description of Gardnerella leopoldii sp. nov., Gardnerella piotii sp. nov. and Gardnerella swidsinskii sp. nov., with Delineation of 13 Genomic Species within the Genus Gardnerella.” Int. J. Syst. Evol. Microbiol. 69 (2019). PubMed: 30648938.
10. Sousa, M., et al. “Gardnerella pickettii sp. nov. (formerly Gardnerella Genomic Species 3) and Gardnerella greenwoodii sp. nov. (Formerly Gardnerella Genomic Species 8) Isolated from Female Urinary Microbiome.” Int. J. Syst. Evol. Microbiol. 73 (2023). PubMed: 37921436.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Gardnerella vaginalis, Strain JCP7275, HM-1105."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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