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Product Name:
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SK119
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Staphylococcaceae, Staphylococcus
Species: Staphylococcus hominis
Strain: SK119
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Additional Information:
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S. hominis is a Gram-positive, non-motile, coagulase-negative staphylococci (CoNS) that is a commensal resident of human skin covering the axillae, head, legs and arms. It is also an opportunistic pathogen in immunocompromised patients and neonates and has been associated as the causal agent of bacteremia, septicemia and endocarditis.2,3 S. hominis is subspeciated into S. hominis subsp. hominis and S. hominis subsp. novobiosepticus based on the combined characteristics of novobiocin resistance and failure to produce acid aerobically from D-trehalose and N-acetyl-D-glucosamine in S. hominis subsp. novobiosepticus.4 The two subspecies also differ in the presence and type of SCCmec cassette they carry. The majority of S. hominis subsp. novobiosepticus isolates have SCCmec cassette components similar to the ones found in S. aureus, whereas only a small subset of S. hominis subsp. hominis isolates carry a SCCmec cassette, and there is a larger diversity of SCCmec components.5
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Brain Heart Infusion broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-119 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Brain Heart Infusion broth or equivalent
Tryptic Soy agar with 5% defibrinated sheep blood or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Perez-Perez, G. I., Personal Communication.
2. Kloos, W. E. and K. H. Schleifer. "Isolation and Characterization of Staphylococci from Human Skin. II. Descriptions of Four New Species: Staphylococcus warneri, Staphylococcus capitis, Staphylococcus hominis, and Staphylococcus simulans." Int. J. Syst. Bacteriol. 25 (1975): 62-79.
3. Chaves, F., et al. “Nosocomial Spread of a Staphylococcus hominis subsp. novobiosepticus Strain Causing Sepsis in a Neonatal Intensive Care Unit.” J. Clin. Microbiol. 43 (2005): 4877-4879. PubMed: 16145165.
4. Mendoza-Olazarán, S., et al. “Microbiological and Molecular Characterization of Staphylococcus hominis Isolates from Blood.” PLoS One 8 (2013): e61161. PubMed: 23585877.
5. Kloos, W. E., et al. “Staphylococcus hominis subsp. novobiosepticus subsp. nov., a Novel Trehalose- and N Acetyl-D-Glucosamine-Negative, Novobiocin- and Multiple-Antibiotic-Resistant Subspecies Isolated from Human Blood Cultures.” Int. J. Syst. Bacteriol. 48 (1998): 799-812. PubMed: 9734034.
6. Zhang, L., et al. “Multilocus Sequence Typing and Further Genetic Characterization of the Enigmatic Pathogen, Staphylococcus hominis.” PLoS One 8 (2013): e66496. PubMed: 23776678.
7. Park, B., T. Iwase and G. Y. Liu. “Intranasal Application of S. epidermidis Prevents Colonization by Methicillin-Resistant Staphylococcus aureus in Mice.” PLoS One 6 (2011): e25880. PubMed: 21998712.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Staphylococcus hominis, Strain SK119, HM-119.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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