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Product Name:
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F0139
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Peptoniphilaceae, Parvimonas1,2
Species: Parvimonas sp.
Subtaxon: Oral Taxon 110
Strain: F0139
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Additional Information:
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Parvimonas species are obligately anaerobic, non-sporulating, Gram-positive cocci that commonly inhabit the human mouth and likely the gastro-intestinal and female genito-urinary tract.4 The only currently recognized species in the genus, Parvimonas micra, is a putative periodontal pathogen, known for involvement in polymicrobial infections of patients with refractory periodontitis.5
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Chopped Meat Carbohydrate medium with 0.1% cellobiose, 0.1% maltose, 0.1% starch and 0.1% Tween 80 supplemented with 5% DMSO.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-207 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Chopped Meat Carbohydrate medium with 0.1% cellobiose, 0.1% maltose, 0.1% starch and 0.1% Tween 80 or equivalent
Tryptic Soy agar with 5% defibrinated sheep blood or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Anaerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 3 to 5 days.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Johnson, C. N., et al. “Peptoniphilus stercorisuis sp. nov., Isolated from a Swine Manure Storage Tank and Description of Peptoniphilaceae fam. nov.” Int. J. Syst. Evol. Microbiol. 64 (2014): 3538-3545. PubMed: 25056296.
2. Tindall, B. J. and J. P. Euzéby. “Proposal of Parvimonas gen. nov. and Quatrionicoccus gen. nov. as Replacements for the Illegitimate, Prokaryotic, Generic Names Micromonas Murdoch and Shah 2000 and Quadricoccus Maszenan et al. 2002, respectively.” Int. J. Syst. Evol. Microbiol. 56 (2006): 2711-2713. PubMed: 17082417.
3. Izard, J., Personal Communication.
4. Murdoch, D. A. and H. N. Shah. "Reclassification of Peptostreptococcus magnus (Prevot 1933) Holdeman and Moore 1972 as Finegoldia magna comb. nov. and Peptostreptococcus micros (Prevot 1933) Smith 1957 as Micromonas micros comb. nov." Anaerobe 5 (1999): 555-559.
5. Colombo, A. P. V., et al. “Comparisons of Subgingival Microbial Profiles of Refractory Periodontitis, Severe Periodontitis, and Periodontal Health Using the Human Oral Microbe Identification Microarray.” J. Periodontol. 80 (2009): 1421-1432. PubMed: 19722792.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Parvimonas sp., Oral Taxon 110, Strain F0139, HM-207."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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