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Product Name:
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KLE1255
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Ruminococcaceae, Faecalibacterium
Species: Faecalibacterium prausnitzii (formerly Fusobacterium prausnitzii)1
Strain: KLE1255
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Additional Information:
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F. prausnitzii is a non-motile, Gram-positive, obligately anaerobic rod that is found in abundance in animal and human feces.1,4,5,6 F. prausnitzii is one of the predominant butyrate-producing bacteria in the gastrointestinal tract and has shown anti-inflammatory effects in probiotic treatment models.7 It is significantly underrepresented in patients with colitis and Crohn’s disease, suggesting the importance of F. prausnitzii in gut homeostasis.8,9
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Modified Chopped Meat broth with carbohydrates and rumen supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-473 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Modified Chopped Meat medium with carbohydrates and rumen or equivalent
Brain Heart Infusion agar with yeast extract and 0.1% cysteine or equivalent 2
Incubation:
Temperature: 37°C
Atmosphere: Anaerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 2 to 3 days.
Note: If the recommended growth conditions do not produce a viable culture the depositor also recommends co-culturing with Escherichia coli (E. coli) or growth in spent medium from E. coli.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Duncan, S. H., et al. "Growth Requirements and Fermentation Products of Fusobacterium prausnitzii and a Proposal to Reclassify it as Faecalibacterium prausnitzii gen. nov., comb. nov." Int. J. Syst. Evol. Microbiol. 52 (2002): 2141-2146. PubMed: 12508881.
2. Witt, K., Personal Communication.
3. Cato, E. P., C. W. Salmon and W. E. C. Moore. "Fusobacterium prausnitzii (Hauduroy et al.) Moore and Holdeman: Emended Description and Designation of Neotype Strain." Int. J. Syst. Bacteriol. 24 (1974): 225-229.
4. Foditsch, C. et al. “Isolation and Characterization of Faecalibacterium prausnitzii from Calves and Piglets.” PLoS One 9 (2014): e116465. PubMed: 25551453.
5. Fitzgerald, C. B., et al. “Comparative Analysis of Faecalibacterium prausnitzii Genomes shows a High Level of Genome Plasticity and Warrants Separation into New Species-Level Taxa.” BMC Genomics 19 (2018): 931. Pubmed: 30547746.
6. Sokol, H., et al. “Faecalibacterium prausnitzii is an Anti-Inflammatory Commensal Bacterium Identified by Gut Microbiota Analysis of Crohn Disease Patients.” Proc. Natl. Acad. Sci. U.S.A. 105 (2008): 16731-16736. PubMed: 18936492.
7. Sokol, H., et al. “Low Counts of Faecalibacterium prausnitzii in Colitis Microbiota.” Inflamm. Bowel Dis. 15 (2009): 1183-1189. PubMed: 19235886.
8. Jia, W., et al. “Is the Abundance of Faecalibacterium prausnitzii Relevant to Crohn's Disease?” FEMS Microbiol. Lett. 310 (2010): 138-144. PubMed: 20695899.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Faecalibacterium prausnitzii, Strain KLE1255, HM-473."
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Biosafety Level:
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1
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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