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Product Name:
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MIT 10-5246 (Deposited as Klebsiella oxytoca, Strain MIT 10-5246)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Enterobacteriaceae, Raoultella
Species: Raoultella ornithinolytica (Previously referred to as Klebsiella oxytoca, this strain has been reclassified.)1
Strain: MIT 10-5246 (also referred to as 10-5246)
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Additional Information:
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R. ornithinolytica is a Gram-negative, non-motile, rod shaped bacterium originally classified as the genus Klebsiella.1,3 It is ubiquitous in the environment and has been isolated from water, soil, insects, fish, ticks and termites.1,3 R. ornithinolytica is an emerging opportunistic pathogen associated with both nosocomial and community-acquired infections, particularly in immunocompromised patients, with an increasing spread of antibiotic-resistance, especially of extended-spectrum β-lactamase (ESBL)-producing strains.3,4 R. ornithinolytica, strain MIT 10-5246 is negative for a cytotoxin that has been associated with hemorrhagic colitis.2
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-627 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Tryptic Soy Broth or equivalent
Tryptic Soy Agar or equivalent
Incubation:
Temperature: 35°C to 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Drancourt, M., et al. “Phylogenetic Analyses of Klebsiella Species Delineate Klebsiella and Raoultella gen. nov., with Description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov.” Int. J. Syst. Evol. Microbiol. 51 (2001): 925-932. PubMed: 11411716.
2. Fox, J. G., Personal Communication.
3. Hajjar, R., et al. “Raoultella ornithinolytica: Emergence and Resistance.” Infect. Drug Resist. 13 (2020): 1091-1104. PubMed: 32346300.
4. Seng, P., et al. “Emerging role of Raoultella ornithinolytica in Human Infections: A Series of Cases and Review of the Literature.” Int. J. Infect. Dis. 45 (2016): 65-71. PubMed: 26921549.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Raoultella ornithinolytica, Strain MIT 10-5246 (Deposited as Klebsiella oxytoca, Strain MIT 10-5246), HM-627."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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