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Product Name:
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MIT 10-5250
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Enterobacteriaceae, Klebsiella
Species: Klebsiella pasteurii (HM-630 was deposited to BEI Resources as Klebsiella oxytoca and later reclassified as Klebsiella michiganensis.1,2 Further reclassification to Klebsiella pasteurii has been proposed.3 Digital DNA-DNA hybridization (dDDH) analysis performed at BEI Resources supports the reclassification of strain MIT 10-5250 to Klebsiella pasteurii.)
Note: The species designation on the vial label refers to the original nomenclature.
Strain: MIT 10-5250 (also referred to as 10-5250)
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Additional Information:
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The K. oxytoca species complex consists of nine members, K. grimontii, K. huaxiensis, K. michiganensis, K. oxytoca, K. pasteurii, K. spallanzanii and three unnamed novel species, each representing a phylogroup characterized by sequence variations within the chromosomally encoded β-lactamase gene, blaOXY.1 K. pasteurii represents phylogroup Ko4 (blaOXY-4).1 The species within the K. oxytoca complex are non-motile, Gram-negative, rod-shaped bacteria that cause frequent nosocomial infections of the urinary and respiratory tracts. They are ubiquitous in the environment and are often isolated from the skin, mucous membranes and intestines of humans and animals.5 Due to the extensive spread of antibiotic-resistant strains, especially of extended-spectrum β-lactamase (ESBL)-producing strains, there has been renewed interest in infections by members of this species complex.6,7
K. pasteurii, strain MIT 10-5250 is reported as positive for a cytotoxin that has been associated with hemorrhagic colitis.4
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-630 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Tryptic Soy Broth or equivalent
Tryptic Soy Agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Saha, R., et al. “Klebsiella michiganensis sp. nov., a New Bacterium Isolated from a Tooth Brush Holder.” Curr. Microbiol. 66 (2013): 72-78. PubMed: 23053492.
2. Euzeby, J. P. “Validation List no. 150. List of New Names and New Combinations Previously Effectively, but Not Validly, Published.” Int. J. Syst. Evol. Microbiol. 63 (2013): 797-798.
3. Yang, J., et al. “Klebsiella oxytoca Complex: Update on Taxonomy, Antimicrobial Resistance, and Virulence.” Clin. Microbiol. Rev. 35 (2022): e0000621. PubMed: 34851134.
4. Fox, J. G., Personal Communication.
5. Podschun, R. and U. Ullmann. “Klebsiella spp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors.” Clin. Microbiol. Rev. 11 (1998): 589-603. PubMed: 9767057.
6. Decré, D., et al. “Outbreak of Multi-resistant Klebsiella oxytoca Involving Strains with Extended-Spectrum β-lactamases and Strains with Extended-Spectrum Activity of the Chromosomal β-lactamase.” J. Antimicrob. Chemother. 54 (2004): 881-888. PubMed: 15472005.
7. Granier, S. A., et al. “Recognition of Two Genetic Groups in the Klebsiella oxytoca Taxon on the Basis of Chromosomal β-lactamase and Housekeeping Gene Sequences as Well as ERIC-1R PCR Typing.” Int. J. Syst. Evol. Microbiol. 53 (2003): 661-668. PubMed: 12807183.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Klebsiella pasteurii, Strain MIT 10-5250 (Deposited as Klebsiella oxytoca, Strain MIT 10-5250), HM-630.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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