| |
|
Product Name:
|
CL03T12C01 (Deposited as Bacteroides dorei, Strain CL03T12C01)
|
|
Manufacturer:
|
BEI Resources
|
|
Taxonomy:
|
Bacteria Classification: Bacteroidaceae, Phocaeicola
Species: Phocaeicola dorei (Previously referred to as Bacteroides dorei, this genus has been reclassified and the designation on the vial label refers to the old nomenclature.)1,2
Strain: CL03T12C01
|
|
Additional Information:
|
P. dorei is a Gram-negative, anaerobic, non-motile bacterium typically isolated from the gastrointestinal tract and feces of humans.4,5 P. dorei has been reported to play a role as immunomodulator in autoimmune diseases and its pathogenic potential has also been reported.6,7
|
|
Material Provided:
|
Each vial contains approximately 0.5 mL of bacterial culture in Modified Reinforced Clostridial broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
|
|
Packing/Storage:
|
HM-718 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
|
|
Growth Conditions:
|
Media:
Modified Reinforced Clostridial broth or Modified Chopped Meat broth or equivalent
Tryptic Soy agar with 5% defibrinated sheep blood or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Anaerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 2 to 4 days.
|
|
Disclaimers:
|
You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
|
1. García-Lopez, M., et al. “Analysis of 1,000 Type-Strain Genomes Improves Taxonomic Classification of Bacteroidetes.” Front. Microbiol. 10 (2019): 2083. PubMed: 31608019.
2. Oren, A. and Garrity, G. M. “List of New Names and New Combinations Previously Effectively, but Not Validly, Published.” Int. J. Syst. Evol. Microbiol. 70 (2020): 2960-2966. PubMed: 32471528.
3. Comstock, L. E., Personal Communication.
4. Bakir, M. A., et al. “Bacteroides dorei sp. nov., Isolated from Human Faeces.” Int. J. Syst. Evol. Microbiol. 56 (2006): 1639-1643. PubMed: 16825642.
5. Sánchez, E., et al. “Intestinal Bacteroides Species Associated with Coeliac Disease.” J. Clin. Pathol. 63 (2010): 1105-1111. PubMed: 20972239.
6. Vatanen, T., et al. “Variation in Microbiome LPS Immunogenicity Contributes to Autoimmunity in Humans.” Cell 165 (2016): 842-853. PubMed: 27133167.
7. Matsuoka, T., et al. “First Case of an Invasive Bacteroides dorei Infection Detected in a Patient with a Mycotic Aortic Aneurysm – Raising a Rebellion of Major Indigenous Bacteria in Humans: A Case Report and Review.” BMC Infect. Dis. 21 (2021): PubMed: 34193073.
8. Wexler, H. M. “Bacteroides: the Good, the Bad, and the Nitty-Gritty.” Clin. Microbiol. Rev. 20 (2007): 593-621. PubMed: 17934076.
9. Pedersen, R. M., E. S. Marmolin and U. S. Justesen. “Species Differentiation of Bacteroides dorei from Bacteroides vulgatus and Bacteroides ovatus from Bacteroides xylanisolvens – Back to Basics.” Anaerobe 24 (2013): 1-3. PubMed: 23994205.
|
|
Citation:
|
Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Bacteroides dorei, Strain CL03T12C01 (Deposited as Bacteroides dorei, CL03T12C01), HM-718."
|
|
Biosafety Level:
|
1
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
|