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Product Name:
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002-P50-2011 (Deposited as Clostridium difficile, Strain 002-P50-2011)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Peptostreptococcaceae, Clostridioides1,2
Species: Clostridioides difficile (Previously referred to as Clostridium difficile, this genus has been reclassified and the designation on the vial label refers to the old nomenclature.)3
Strain: 002-P50-2011
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Additional Information:
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C. difficile is a Gram-positive, spore-forming, obligate anaerobe that commonly inhabits the intestinal tract of various mammalian species, reptiles and birds, and may also be found in the environment. Pathogenic strains of C. difficile produce a potent cytotoxin (toxin B) and in most cases an enterotoxin (toxin A).5 It is the production of these toxins in the gut which ultimately leads to the disease pseudomembranous colitis (PMC) and C. difficile associated diarrhea (CDAD), which often occur as a complication of antibiotic therapy in elderly hospitalized patients.6
C. difficile, strain 002-P50-2011 tests positive for virulence toxin B by PCR and toxins A and B by enzyme immunoassay and Western blot analysis, is noncytotoxic in cell culture and mouse inoculation studies, is a spore-former, and did not induce P-MK2 signaling molecule in MAPKAP2 induction studies.1
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Modified Reinforced Clostridial broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-746 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Modified Reinforced Clostridial broth or equivalent
Tryptic Soy agar with 5% defibrinated sheep blood or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Anaerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Oren, A. and G. M. Garrity. “List of New Names and New Combinations Previously Effectively, but not Validly, Published.” Int. J. Syst. Evol. Microbiol. 66 (2016): 3761-3764. PubMed: 27902176.
2. Yutin, N. and M. Y. Galperin. “A Genomic Update on Clostridial Phylogeny: Gram-Negative Spore-Formers and Other Misplaced Clostridia.” Environ. Microbiol. 15 (2013): 2631-2641. PubMed: 23834245.
3. Lawson, P. A., et al. “Reclassification of Clostridium difficile as Clostridioides difficile (Hall and O'Toole 1935) Prévot 1938.” Anaerobe 40 (2016): 95-99. PubMed: 27370902.
4. Bobo, L. D., Personal Communication.
5. Rupnik, M., M. H. Wilcox and D. N. Gerding. “Clostridium difficile Infection: New Developments in Epidemiology and Pathogenesis.” Nat. Rev. Microbiol. 7 (2009): 526-536. PubMed: 19528959.
6. Kelly, C. P. and J. T. LaMont. “Clostridium difficile – More Difficult than Ever.” N. Engl. J. Med. 359 (2008): 1932-1940. PubMed: 18971494.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Clostridioides difficile, Strain 002-P50-2011 (Deposited as Clostridium difficile, Strain 002-P50-2011), HM-746."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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