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Product Name:
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D9 (Deposited as Shigella sp., Strain D9)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Enterobacteriaceae, Escherichia
Species: Escherichia coli (Previously referred to as Shigella sp., this strain has been reclassified.)
Strain: D9 (also referred to as strain 36_3_1A)
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Additional Information:
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E. coli is a Gram-negative, rod-shaped bacterium commonly found in the gut flora of warm-blooded animals and is the primary facultative anaerobe of the human gastrointestinal tract. While most E. coli strains are harmless and are an important part of a healthy intestinal tract, some serotypes are pathogenic, causing diarrhea, urinary tract infections, respiratory illness, pneumonia or other illnesses in their host.2,3,4 Pathogenic E. coli may be transmitted through contaminated food or water or through contact with infected persons or animals. The six pathotypes associated with diarrhea and collectively referred to as diarrheagenic E. coli are: Shiga toxin-producing E. coli [STEC; also referred to as Verocytotoxin-producing E. coli (VTEC) or enterohemorrhagic E. coli (EHEC)], enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enteroinvasive E. coli (EIEC) and diffusely adherent E. coli (DAEC).5,6,7,8,9
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-87 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Tryptic Soy broth or equivalent
Tryptic Soy Agar with 5% defibrinated sheep blood or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Allen-Vercoe, E., Personal Communication.
2. Nataro, J. P. and J. B. Kaper. “Diarrheagenic Escherichia coli.” Clin. Microbiol. Rev. 11 (1998): 142-201. PubMed: 9457432.
3. Kaper, J. B., J. P. Nataro and H. L. Mobley. “Pathogenic Escherichia coli.” Nat. Rev. Microbiol. 2 (2004): 123-140. PubMed: 15040260.
4. Croxen, M. A., et al. “Recent Advances in Understanding Enteric Pathogenic Escherichia coli.” Clin. Microbiol. Rev. 26 (2013): 822-880. PubMed: 24092857.
5. Smith, J. L., P. M. Fratamico and N. W. Gunther, 4th. “Shiga Toxin-Producing Escherichia coli.” Adv. Appl. Microbiol. 86 (2014): 145-197. PubMed: 24377855.
6. Zhang, W. and D. A. Sack. “Progress and Hurdles in the Development of Vaccines against Enterotoxigenic Escherichia coli in Humans.” Expert Rev. Vaccines 11 (2012): 677-694. PubMed: 22873126.
7. Ochoa, T. J. and C. A. Contreras. “Enteropathogenic Escherichia coli Infection in Children.” Curr. Opin. Infect. Dis. 24 (2011): 478-483. PubMed: 21857511.
8. Estrada-Garcia, T. and F. Navarro-Garcia. “Enteroaggregative Escherichia coli Pathotype: A Genetically Heterogeneous Emerging Foodborne Enteropathogen.” FEMS Immunol. Med. Microbiol. 66 (2012): 281-298. PubMed: 22775224.
9. Smith, E. J., et al. “Pathogenesis of Adherent-Invasive Escherichia coli.” Future Microbiol. 8 (2013): 1289-1300. PubMed: 24059919.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Escherichia coli, Strain D9 (Deposited as Shigella sp., Strain D9), HM-87.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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