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NR-12159  Salmonella enterica subsp. enterica, 14028s ssrAB::kanr (Serovar Typhimurium)
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Organism: Salmonella enterica subsp. enterica
Designations: 14028s ssrAB::kanr (Serovar Typhimurium)
Biosafety Level: 2
Availability Status: Discontinued (Please See Comments)
Store at: -60°C or colder
Contributor: F Heffron
NR-12159, Salmonella enterica subsp. enterica, Strain 14028s ssrAB::kanr (Serovar Typhimurium, is no longer available.

Salmonella enterica (S. enterica) subsp. enterica, strain 14028s ssrAB::kanr (serovar Typhimurium) was derived from strain 14028s (strain 14028s was originally known as strain 14028, however, a variant of the original strain with a rough colony morphology was designated 14028r and the original smooth strain was renamed 14028s). Strain 14028 is a descendent of strain CDC 6516-60 which was isolated from pooled hearts and livers of 4-week-old chickens.

The ssrAB::kanr mutant of strain 14028s was produced by creating a PCR product with ssrAB homologous sequences at the 5' and 3' ends of a linear fragment containing a kanamycin resistance cassette. S. enterica subsp. enterica, strain 14028s was transformed, and insertion of the kan cassette in place of ssrAB was confirmed by PCR. The complete genome (GenBank: CP001363.1) and plasmid (GenBank: CP001362.1) sequences are available.

Each vial contains approximately 0.5 mL of bacterial culture in 0.5X Tryptic Soy broth supplemented with 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Salmonella enterica subsp. enterica, Strain 14028s ssrAB::kanr (Serovar Typhimurium), NR-12159."
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