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NR-12172  Salmonella enterica subsp. enterica, 14028s ΔhimD (Serovar Typhimurium)
(Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Salmonella enterica subsp. enterica
Designations: 14028s ΔhimD (Serovar Typhimurium)
Biosafety Level: 2
Availability Status: Discontinued (Please See Comments)
Store at: -60°C or colder
Contributor: F Heffron
Comments:
NR-12172, Salmonella enterica subsp. enterica, Strain 14028s ΔhimD (Serovar Typhimurium), is no longer available.

Salmonella enterica (S. enterica) subsp. enterica, strain 14028s ΔhimD (serovar Typhimurium) was derived from strain 14028s (strain 14028s was originally known as strain 14028, however, a variant of the original strain with a rough colony morphology was designated 14028r and the original smooth strain was renamed 14028s). Strain 14028 is a descendent of strain CDC 6516-60 which was isolated from pooled hearts and livers of 4-week-old chickens.

The ΔhimD mutant of strain 14028s was produced by creating a PCR product with himD homologous sequences at the 5' and 3' ends of a linear fragment containing a kanamycin resistance cassette. S. enterica subsp. enterica, strain 14028s was transformed, and insertion of the kan cassette in place of himD was confirmed by PCR. The final non-polar deletions were constructed by elimination of the kan cassette. The complete genome (GenBank: CP001363.1) and plasmid (GenBank: CP001362.1) sequences are available.

Each vial contains approximately 0.5 mL of bacterial culture in 0.5X Tryptic Soy broth supplemented with 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Salmonella enterica subsp. enterica, Strain 14028s ΔhimD (Serovar Typhimurium), NR-12172."
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