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Product Name:
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B31 (Clone 5A1)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Borreliaceae (previously Spirochaetaceae), Borrelia1
Species: Borrelia burgdorferi
Strain: B31 (clone 5A1)
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Additional Information:
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B. burgdorferi is a Gram-negative, motile spirochete.5 It is a zoonotic, vector-borne pathogen transmitted by ticks and the etiologic agent of Lyme disease, now the most common tick-transmitted disease in the United States.4 B. burgdorferi is predominant in North America, but also exists in Europe.
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Revised Barbour-Stoenner-Kelly broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-13251 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Revised Barbour-Stoenner-Kelly broth (Appendix) or agar or equivalent
Note: Medium should be prepared fresh before each use.
Incubation:
Temperature: 32°C to 34°C
Note: Growth at 37°C may result in plasmid loss.3
Atmosphere: Microaerophilic
Note: Slower growth occurs under aerobic conditions.3
Propagation:
1. Keep vial in dry ice during inoculations.
2. Inoculate new cultures from scraping of frozen stock into a single tube of Revised BSK Medium.
3. Incubate the tube at 32°C to 34°C for 2 to 14 days. Do not shake culture during growth.
Note: Subculturing should be minimized to avoid plasmid loss.3,6
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use.
Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org.
While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate.
This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products.
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Patents or other restrictions:
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This material may be subject to third party patent rights.
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References:
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1. Gupta, R. S., S. Mahmood and M. Adeolu. “A Phylogenomic and Molecular Signature Based Approach for Characterization of the Phylum Spirochaetes and Its Major Clades: Proposal for a Taxonomic Revision of the Phylum.” Front. Microbiol. 4 (2013): 217. Erratum in: Front. Microbiol. 4 (2013): 322. PubMed: 23908650.
2. Burgdorfer, W., et al. “Lyme Disease – A Tick-Borne Spirochetosis?” Science 216 (1982): 1317-1319. PubMed: 7043737.
3. Norris, S. J., Personal Communication.
4. Fraser, C. M., et al. “Genomic Sequence of a Lyme Disease Spirochaete, Borrelia burgdorferi.” Nature 390 (1997): 580-586. PubMed: 9403685.
5. Johnson, R. C., et al. “Borrelia burgdorferi sp. nov.: Etiologic Agent of Lyme Disease.” Int. J. Syst. Bacteriol. 34 (1984): 496-497.
6. Purser, J. E. and S. J. Norris. "Correlation between Plasmid Content and Infectivity in Borrelia burgdorferi." Proc. Natl. Acad. Sci. USA 97 (2000): 13865-13870. PubMed: 11106398.
7. Barbour, A. G. “Isolation and Cultivation of Lyme Disease Spirochetes.” Yale J. Biol. Med. 57 (1984): 521-525. PubMed: 6393604.
8. Kawabata, H., S. J. Norris and H. Watanabe. “BBE02 Disruption Mutants of Borrelia burgdorferi B31 Have a Highly Transformable, Infectious Phenotype.” Infect. Immun. 72 (2004): 7147-7154. PubMed: 15557639.
9. Casjens, S., et al. “A Bacterial Genome in Flux: The Twelve Linear and Nine Circular Extrachromosomal DNAs in an Infectious Isolate of the Lyme Disease Spirochete Borrelia burgdorferi.” Mol. Microbiol. 35 (2000): 490-516. PubMed: 10672174.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Borrelia burgdorferi, Strain B31 (Clone 5A1), NR-13251."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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