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NR-14  Francisella tularensis subsp. holarctica, 15 (Gaisky Live Vaccine Strain)
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at no cost to registered researchers.
Organism: Francisella tularensis subsp. holarctica
Designations: 15 (Gaisky Live Vaccine Strain)
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: ATCC®
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Francisella tularensis (F. tularensis) subsp. holarctica, strain 15 was isolated from a water vole (Arvicola terrestris) by Gaisky in Russia (1936), where it was used as a live vaccine. Strain 15 was transferred from the Institute of Epidemiology and Microbiology (Gamaleia Institute) to the U. S. Army Medical Research Institute for Infectious Diseases (USAMRIID) in 1956.

F. tularensis subsp. holarctica, strain 15 was deposited to the ATCC ® in 1977 by J. Frederick Bell of the U.S. Public Health Service, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratory in Montana. At the time of deposition this strain was known to produce two colony types (grey/black and blue variants) on blood agar plates and was considered a very attenuated strain of F. tularensis subsp. holarctica. F. tularensis subsp. holarctica, Live Vaccine Strain (LVS) was produced from five passages of the blue colony variant in mice and is available as BEI Resources NR-646.

NR-14 has been confirmed as subsp. holarctica (Type B) by PCR amplification of a subspecies-specific sequence of approximately 1250 bp from extracted DNA.

Each vial contains approximately 0.5 mL of bacterial culture in 0.5X Tryptic Soy Broth supplemented with 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Francisella tularensis subsp. holarctica, Strain 15 (Gaisky Live Vaccine Strain), NR-14."
Publications Citing
this Reagent:
Ahmad, S., et al. "Azithromycin Effectiveness against Intracellular Infections of Francisella." BMC Microbiol. 10 (2010): 123. PubMed: 20416090.
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Derived from:  ATCC 29684
Genomic DNA from Francisella tularensis subsp. holarctica, Strain 15 (Gaisky Live Vaccine Strain)
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