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Product Name:
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16M (NCTC 10094)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Brucellaceae, Brucella
Agent: Brucella melitensis
Biotype/Biovar: 1
Type Strain: 16M (NCTC 10094)
(Derived from ATCC® 23456™)
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Additional Information:
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Brucella species are the etiological agents of brucellosis, a zoonotic disease endemic in many areas of the world and characterized by chronic infections in animals leading to abortion and infertility. The host-pathogen associations between the classical Brucella species are: B. abortus, cattle; B. suis, swine; B. melitensis, goats; B. ovis, sheep; B. canis, dogs and B. neotomae, desert wood rats. Transmission from animal to human via contact with infected animal products or through the air may lead to Malta (or undulant) fever, a long debilitating disease treatable by a prolonged course of antibiotics. Brucella species are recognized as potential agricultural, civilian, and military bioterrorism agents.2
B. melitensis is a non-motile, aerobic, gram-negative coccobacillus which displays the highest degree of human virulence among Brucella species. Very little is known about the genetics of Brucella virulence, largely due to a lack of classical virulence factors. A type IV secretion system has been identified as essential for intracellular survival and multiplication of Brucella.3
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Material Provided:
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Each vial contains approximately 1 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-256 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Tryptic Soy broth
Tryptic Soy agar, or equivalent
Incubation:
Temperature: 30°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use; thaw slowly.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tubes and plate at 30°C for 2 days.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. DelVecchio, V. G., et al. “The Genome Sequence of the Facultative Intracellular Pathogen Brucella melitensis.” Proc. Natl. Acad. Sci. USA 99 (2002): 443-448. PubMed: 11756688. GenBank: AE008917 and AE008918.
2. Halling, S. M., et al. “Completion of the Genome Sequence of Brucella abortus and Comparison to the Highly Similar Genomes of Brucella melitensis and Brucella suis.” J. Bacteriol. 187 (2005): 2715-2726. PubMed: 15805518.
3. Boschiroli, M. L., et al. “Type IV Secretion and Brucella Virulence.” Vet. Microbiol. 90 (2002): 341-348. PubMed: 12414154.
4. Moreno, E. and I. Moriyon. “Brucella melitensis: a Nasty Bug with Hidden Credentials for Virulence.” Proc. Natl. Acad. Sci. USA 99 (2002): 1-3. PubMed: 11782541.
5. Eschenbrenner, M., et al. “Comparative Proteome Analysis of Brucella melitensis Vaccine Strain Rev 1 and a Virulent Strain, 16M.” J. Bacteriol. 184 (2002): 4962-4970. PubMed: 12193611.
6. Chain, P. S. et al. “Whole-Genome Analyses of Speciation Events in Pathogenic Brucellae.” Infect. Immun. 73 (2005): 8353-8361. PubMed: 16299333.
7. Ratushna, V. G., et al. “Molecular Targets for Rapid Identification of Brucella spp.” BMC Microbiol. 6 (2006): 13. PubMed: 16504063.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Brucella melitensis, Strain 16M (NCTC 10094), NR-256."
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Biosafety Level:
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3
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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