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Product Name:
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R038b (deposited as Helicobacter pylori, Strain R038b)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Helicobacteraceae, Helicobacter
Species: Helicobacter sp. (NR-43734 was deposited to BEI Resources as Helicobacter pylori; however, digital DNA-DNA hybridization (dDDH) analysis performed at BEI Resources resulted in reclassification to Helicobacter sp. The designation on the vial label refers to the old nomenclature.)
Strain: R038b
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Additional Information:
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Helicobacter is a genus of Gram-negative, nonsporulating, spiral-shaped and flagellated bacilli consisting of over 50 species commonly found in the human stomach and upper gastrointestinal tract. Helicobacter pylori, the type strain of the genus, is present in about half of the world population.3,4 It is an opportunistic pathogen linked to diseases of the upper gastrointestinal tract, including gastric and duodenal ulcers, chronic gastritis and stomach cancer.2 H. pylori infections are difficult to cure, and successful treatment generally requires the administration of several antibacterial agents simultaneously.5,6
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Brucella broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-43734 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Tryptic Soy broth or Brain Heart Infusion broth or Brucella broth or equivalent
Tryptic Soy agar or Tryptic Soy agar with 5% defibrinated sheep blood or Brucella agar or Columbia agar with 7% defibrinated horse blood, 5 µg/mL trimethoprim, 5 µg/mL vancomycin, 10 µg/mL cefsulodin and 2.5 µg/mL amphotericin B1 or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Microaerophilic (~ 6-16% O2 and 2-10% CO2)
Propagation:
1. Keep the vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 2 to 3 days.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
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Patents or other restrictions:
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This material may be subject to third party rights.
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References:
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1. Blanchard, T.G., Personal Communication.
2. Blanchard, T.G., et al. “Genome Sequences of 65 Helicobacter pylori Strains Isolated from Asymptomatic Individuals and Patients with Gastric Cancer, Peptic Ulcer Disease, or Gastritis.” Pathog. Dis. 68 (2013): 39-43. PubMed: 23661595.
3. Cover, T. L. and M. J. Blaser. “Helicobacter pylori in Health and Disease.” Gastroenterology 136 (2009): 1863-1873. PubMed: 19457415.
4. Tomb, J. F., et al. “The Complete Genome Sequence of the Gastric Pathogen Helicobacter pylori.” Nature 388 (1997): 539-47. PubMed: 9252185.
5. Graham, D. Y., H. Lu and Y. Yamaoka. “Therapy for Helicobacter pylori Infection Can Be Improved: Sequential Therapy and Beyond.” Drugs 68 (2008): 725-736. PubMed: 18416582.
6. Graham, D. Y. and L. Fischbach. “Helicobacter pylori Treatment in the Era of Increasing Antibiotic Resistance.” Gut 59 (2010): 1143-1153. PubMed: 20525969.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Helicobacter sp., Strain R038b (deposited as Helicobacter pylori, Strain R038b, NR-43734."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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