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Product Name:
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2285 Rough
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Mycobacteriaceae, Mycobacterium
Species: Mycobacterium avium
Strain: 2285 Rough
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Additional Information:
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M. avium is an acid-fast, Gram-positive, non-motile, nonchromogenic, slow-growing bacillus ubiquitous in a number of environmental sources including water, soil and plants.2 This opportunistic pathogen is capable of causing disease in both humans and animals. M. avium is subspeciated into M. avium subsp. avium, M. avium subsp. hominissuis, M. avium subsp. paratuberculosis and M. avium subsp. silvaticum, each of which has a specific host or hosts but shares many genotypic and phenotypic features.3,4,5
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Middlebrook 7H9 broth with ADC enrichment supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-44264 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Middlebrook 7H9 broth with ADC enrichment or equivalent
Middlebrook 7H10 agar with OADC enrichment or Lowenstein Jensen agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic with 5% CO2
Propagation:
1. Keep vial frozen until ready for use; then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tubes and plate at 37°C for 2 to 6 weeks.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Ordway, D., Personal Communication.
2. Inderlied, C. B., C. A. Kemper and L. E. Bermudez. “The Mycobacterium avium Complex.” Clin. Microbiol. Rev. 6 (1993): 266-310. PubMed: 8358707.
3. Thorel, M. F., M. Krichevsky and V. V. Levy-Frebault. "Numerical Taxonomy of Mycobactin-Dependent Mycobacteria, Emended Description of Mycobacterium avium, and Description of Mycobacterium avium subsp. avium subsp. nov., Mycobacterium avium subsp. paratuberculosis subsp. nov., and Mycobacterium avium subsp. silvaticum subsp. nov." Int. J. Syst. Bacteriol. 40 (1990): 254-260. PubMed: 2397193.
4. Turenne, C. Y., R. Wallace, Jr. and M. A. Behr. “Mycobacterium avium in the Postgenomic Era.” Clin. Microbiol. Rev. 20 (2007): 205-229. PubMed: 17428883.
5. Mackenzie, N., et al. “Genomic Comparison of PE and PPE Genes in the Mycobacterium avium Complex.” J. Clin. Microbiol. 47 (2009): 1002-1011. PubMed: 19144814.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium avium, Strain 2285 Rough, NR-44264.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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